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KMID : 0545120170270010057
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Journal of Microbiology and Biotechnology
2017 Volume.27 No. 1 p.57 ~ p.66
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Aflatoxin B1 Detoxification by Aspergillus oryzae from Meju, a Traditional Korean Fermented Soybean Starter
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Lee Kyu-Ri
Yang Sun-Min
Cho Sung-Min
Kim Myung-Hee
Hong Sung-Yong
Chung Soo-Hyun
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Abstract
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Aflatoxins are classified as Group 1 (carcinogenic to humans) by the International Agency for Research on Cancer. In this study, a total of 134 fungal strains were isolated from 65 meju samples, and two fungal isolates were selected as potential aflatoxin B1 (AFB1)- biodetoxification fungi. These fungi were identified as Aspergillus oryzae MAO103 and A. oryzae MAO104 by sequencing the beta-tubulin gene. The two A. oryzae strains were able to degrade more than 90% of AFB1 (initial concentration: 40 μg/l) in a culture broth in 14 days. The mutagenic effects of AFB1 treated with A. oryzae MAO103 and MAO104 significantly decreased to 5.7% and 6.4%, respectively, in the frame-shift mutation of Ames tests using Salmonella typhimurium TA98. The base-substituting mutagenicity of AFB1 was also decreased by the two fungi. Moreover, AFB1 production by Aspergillus flavus was significantly decreased by the two A. oryzae strains on soybean-based agar plates. Our data suggest that the two AFB1- detoxifying A. oryzae strains have potential application to control AFB1 in foods and feeds.
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KEYWORD
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Aflatoxin B1, biodegradation, detoxification, soybean fermentation starter
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